Monitoring wool fibre diameter and length changes using gel
emulsion autoradiography
M.A. Friend, G.E. Robards
Department of Wool and Animal Science, University of New South
Wales, Sydney NSW 2052 Australia
Proceedings of the New Zealand Society of Animal Production. 1995,
55: 28-30
A technique for radio-labelling wool with using 35S(cysteine) was
modified by using a gel emulsion photographic film. The use of gel
photographic emulsion rather than sheet x-ray film reduces labour input
and produces clearer autoradiographs. The autoradiographs produced are
ideal for fibre diameter measurement at specific points in time, and
for fibre length measurements between specific times. The adequacy of
labelling using an intradermal injection was also studied. Provided
that the area delineated for injection was not too large, adequate
labelling of fibres was achieved. The use of a specific intradermal
injection, rather than a systemic intravenous injection greatly reduces
the cost of the procedure
Keywords: NZSAPAB;
Autoradiography; fibre diameter; Merino sheep
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Last Updated 25-01-1997
